Universal Evolutionary Trace

ET color scale
More important Prismatic/gobstopper color scale of evolutionary importance

Less important
ET mapped onto the structure

ET mapped onto the sequenceHover your mouse pointer over the amino acid symbol to see the residue numberClick on the amino acid letter and the corresponding residue will be highlighted on the structure as spacefill(spheres)

PAEKRKPIRV LSLFDGIATG LLVLKDLGIQ VDRYIASEVC EDSITVGMVR HQGKIMYVGD 

VRSVTQKHIQ EWGPFDLVIG GSPCNDLSIV NPARKGLYEG TGRLFFEFYR LLHDARPKEG 

DDRPFFWLFE NVVAMGVSDK RDISRFLESN PVMIDAKEVS AAHRARYFWG NLPGMNRPLA 

STVNDKLELQ ECLEHGRIAK FSKVRQHFPV FMNEKEDILW CTEMERVFGF PVHYTDVSNM 

SRLARQRLLG RSWSVPVIRH LFAPLKEYFA CV
From  To

You may also use PyMOL after installing the PyETV PyMOL pluginPre-computed ET analysis can be downloaded directly into PyMOL using PyETV

All data files	2qrvA.zip
ET ranks file	ET_2qrvA.ranks
Multiple sequence alignment	2qrvA.msf (97 sequences)
Click image to download a PyMOL session file

Sequence identity treeHover your mouse pointer over the sequence name to see the source organism name

You may choose to run a new trace using the form below:

Show Advanced Options

Warning: Running a new trace may start a BLAST search followed by alignment of the sequences, which may take up to several minutes.

Questions? Contact us.

Advanced Input and Parameters (Optional)
Trace Options
Real-Valued Trace (rvET)
Optimize selection of sequences from alignment for rvET
Position-specific gap-reducing real-valued trace
pair-interaction ET (piET)
BLAST
E-value cutoff:
Number of proteins to extract:
Use PSI-BLAST
Number of PSI-BLAST iterations:
Use all sequences from all iterations of PSI-BLAST
BLAST Database Options
Custom NCBI
Custom UniRef90
Custom UniRef100
Custom SwissProt reviewed
Sequence Selection
Restrict BLAST hits to gi#'s:
Restrict BLAST hits to # proteins:
Restrict BLAST hits to minimum e-value:
Restrict BLAST hits to maximum e-value:
Restrict BLAST hits to minimum sequence identity:
Restrict BLAST hits to maximum sequence identity:
Restrict BLAST hits to minimum fractional length to query:
Do not remove fragments
Fractional length used to define fragments (in relation to the query in the alignment):
Do not remove identical sequences
Do not remove short sequences
Fractional length used to define short sequences (in relation to the query):

Advanced Input and Parameters (Optional)
Trace Options
Real-Valued Trace (rvET)
Optimize selection of sequences from alignment for rvET
Position-specific gap-reducing real-valued trace
pair-interaction ET (piET)

Alignment File (GCG/MSF format):
-Or- Sequences File (FASTA format):
Query name in input file (eg: query_1aca):
Tree File (NHX format)(Optional):